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目的检测Fra-1在肝细胞性肝癌(简称肝癌)及癌旁组织中的表达情况,探讨其与肝癌病理特征间的关系。方法采用免疫组化法检测Fra-1在60例肝癌及癌旁组织中的表达情况;采用Western blot、实时定量PCR(qRT-PCR)法检测Fra-1在20对冰冻肝癌及癌旁组织中的相对表达量。结果免疫组化示Fra-1在肝癌组织细胞核中阳性表达率为57%(34/60),细胞质中未见明显表达;而Fra-1在癌旁组织细胞质阳性表达率为23%(14/60),细胞核中未见明显表达。Fra-1在肝癌组织中的表达和血清甲胎蛋白(sAFP)、肿瘤血管侵犯相关(χ~2=4.538、9.086,P<0.05),而与肝癌患者的肿瘤包膜、肝癌TNM分期、Edmondson分级等其他临床病理特征无明显相关性;Western blot及qRT-PCR检测结果示肝癌组织中Fra-1的相对表达量高于癌旁组织(P<0.01)。结论 Fra-1与sAFP及肿瘤血管侵犯相关,提示Fra-1可能参与调控肝癌甲胎蛋白(AFP)的生成并影响肝癌的发展;为肝癌的诊治提供一些新的思路及理论基础。
Objective To detect the expression of Fra-1 in hepatocellular carcinoma (HCC) and its adjacent tissues, and to explore its relationship with the pathological features of HCC. Methods Immunohistochemistry was used to detect the expression of Fra-1 in 60 cases of hepatocellular carcinoma and its adjacent tissues. Western blot and real-time quantitative PCR (qRT-PCR) were used to detect the expression of Fra-1 in 20 pairs of frozen hepatocellular carcinoma and its adjacent tissues Relative expression level. Results The positive expression rate of Fra-1 in the nucleus of HCC tissues was 57% (34/60), but not in the cytoplasm. The positive expression rate of Fra-1 in para-cancerous tissues was 23% (14 / 60), no obvious expression in the nucleus. The expression of Fra-1 in hepatocellular carcinoma was correlated with serum alpha-fetoprotein (sAFP) and tumor vascular invasion (χ ~ 2 = 4.538,9.086, P <0.05) There was no significant correlation between the expression of Fra-1 and other clinical and pathological features. Western blot and qRT-PCR showed that the relative expression of Fra-1 in hepatocellular carcinoma was higher than that in paracancerous tissues (P <0.01). Conclusion Fra-1 is associated with sAFP and tumor vascular invasion, suggesting that Fra-1 may be involved in the regulation of AFP formation and the development of hepatocellular carcinoma. It may provide some new ideas and theoretical basis for the diagnosis and treatment of hepatocellular carcinoma.