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目的应用高效液相色谱法对龙爪槐角与槐角对照药材中主要的黄酮苷元成分进行比较,探索龙爪槐角替代国槐槐角应用的可行性。方法采摘石家庄不同地点的龙爪槐角,以槐角对照药材为参照,提取水解后,高效液相色谱法绘制HPLC色谱图,同时测定其中的主要黄酮苷元成分槲皮素、山柰酚和主要异黄酮苷元染料木素的含量。HPLC测定条件:Diamonsil C18柱(150mm×4.6mm,5μm),0.4%磷酸-甲醇(50∶50)为流动相,流速1.0ml/min,检测波长360nm。结果龙爪槐角与槐角对照药材HPLC色谱图峰型基本一致,其中的槲皮素、染料木素和山柰酚平均含量分别为(0.30±0.07)%、(3.04±0.27)%和(2.74±0.30)%,与槐角对照药材中三者的含量接近。结论龙爪槐角与槐角对照药材的主要黄酮苷元成分种类相同,含量接近,具有代替槐角的应用前景。
Objective To compare the main components of flavonoid aglycone in Radix Alopecuroides and Angelica sinensis by high performance liquid chromatography (HPLC), and to explore the feasibility of using Radix acuminata as an alternative to Guojiaojiao. Methods Extracted from different parts of Shijiazhuang, Dragon’s Toad, and referenced to the reference material of Acer horn, extracted and then hydrolyzed, HPLC chromatograms were drawn and the main flavonoid aglycones quercetin, kaempferol and its components were determined. The main isoflavone aglycone genistein content. HPLC measurement conditions: Diamonsil C18 column (150 mm×4.6 mm, 5 μm), 0.4% phosphoric acid-methanol (50:50) as mobile phase, flow rate 1.0 ml/min, detection wavelength 360 nm. Results The peak chromatograms of the HPLC chromatograms of Radix Alopecuroides were similar to those of the control. The average content of quercetin, genistein and kaempferol were (0.30±0.07)%, (3.04±0.27)%, and ( 2.74±0.30)%, which is similar to the content of the three herbs in the control medicines of horns. Conclusion The main flavonoid aglycone components of Radix Alopecuroides and Radix Angelicae sinensis are the same, and their contents are close, which has the application prospect of replacing the horn.