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新近发现α1,3岩藻糖基转移酶 (FUT9)参与LeX 寡糖的合成。通过逆转录 聚合酶链式反应、免疫印迹分析和免疫组化等方法 ,研究了在不同激素状态下人子宫内膜FUT9基因的表达 ,以及LeX 寡糖表达的调控机制。结果显示 :FUT9基因在人子宫内膜中表达 ,且其mRNA在分泌期的表达水平高于增生期 ,口服米非司酮(mifepristone)后 ,增生期内膜的表达量增加 ;LeX 寡糖主要分布在人子宫内膜腔上皮和腺上皮细胞表面 ,在不同生理时期其变化趋势与FUT9基因相似 ,并且口服米非司酮后 ,分泌期内膜的表达量减少 ;在内膜中检测到 3种(33kD、35kD和 85kD)LeX 寡糖蛋白 ,口服米非司酮后 35kD的LeX 寡糖蛋白消失。结果表明 :FUT9基因在人子宫内膜中有明确表达 ,且其表达受卵巢激素的调节 ,孕激素对其有上调作用 ;卵巢激素在转录水平上实现对LeX 寡糖表达的调节。
It was recently found that α1,3 fucosyltransferase (FUT9) is involved in the synthesis of LeX oligosaccharides. The expression of FUT9 gene in human endometrium and the regulation of LeX oligosaccharide expression under different hormone status were studied by reverse transcription polymerase chain reaction, Western blot analysis and immunohistochemistry. The results showed that: FUT9 gene was expressed in human endometrium, and the expression level of mRNA in secretory phase was higher than that in proliferative phase. After oral administration of mifepristone, the expression of FUT9 gene increased in endometrium; Distributed in human endometrial epithelial cells and glandular epithelial cell surface at different physiological stages of its tendency to change with FUT9 gene similar, and oral mifepristone, secretory endometrial expression decreased; detected in the intima 3 (33 kD, 35 kD, and 85 kD) LeX oligosaccharide protein, 35 kD of LeX oligosaccharide protein disappeared after oral administration of mifepristone. The results showed that FUT9 gene was expressed in human endometrium, and the expression of FUT9 gene was regulated by ovarian hormones, which was up-regulated by progesterone. Ovarian hormones could regulate the expression of LeX oligosaccharides at the transcriptional level.