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本实验采用同位素示踪法以及竞争性蛋白结合法,对大鼠心肌缺血10min,再灌30s~(46)Ca内流和cAMP含量变化与再灌心律失常关系,以及牛磺酸(Taur),硫酸镁(MgSO_4)抗再灌心律失常与影响心肌~(45)Ca内流和cAMP含量关系进行了研究.结果表明,心肌缺血10_(min)再灌30s~(45)Ca内流明显高于正常和缺血心肌.Taur,MgSO_4单用及合用均能降低再灌心律失常发生率和抑制再灌心肌~(45)Ca,内流,两药合用更为明显。心肌缺血10min再灌30s cAMP含量未见变化,Taur,MgSO_4亦对其未见影响。证实两药抗再灌心律失常机制与抑制Ca~(2+)内流,防止“钙超负荷”相关。
In this study, we used isotope tracer and competitive protein binding assay to study the relationship between influx and cAMP content in myocardial ischemia 10 min, reperfusion 30 s ~ (46) Ca and arrhythmia of reperfusion, as well as taurine (Taur) , Magnesium sulfate (MgSO_4), anti-reperfusion arrhythmia and myocardial infiltration of ~ (45) Ca influx and cAMP content were studied. The results showed that myocardial ischemia 10 min reperfusion 30s 45 influx Higher than normal and ischemic myocardium.Taur, MgSO_4 alone and in combination can reduce the incidence of reperfusion arrhythmia and inhibit myocardial reperfusion 45 ~ Ca, influx, the combination of the two drugs is more obvious. Myocardial ischemia 10min 30s cAMP content showed no change, Taur, MgSO_4 also had no effect on it. Confirmed that the two anti-reperfusion arrhythmia mechanism and inhibition of Ca ~ (2+) influx, to prevent “calcium overload” related.