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目的研究金钗石斛多糖对四氯化碳复合乙醇所致大鼠肝纤维化的保护作用及可能机制。方法 SD大鼠随机分为正常组、模型组、西药组、中成药组、石斛多糖小剂量组、石斛多糖中剂量组、石斛多糖大剂量组,除正常组外其他组采用四氯化碳复合乙醇诱导大鼠肝纤维化模型,造模8周成功后,分别给予相应药物进行干预,每日1次,连续4周,正常组、模型组给予生理盐水。采用HE染色观察肝组织纤维化程度,检测大鼠血清中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、超氧化物歧化酶(SOD)、丙二醛(MDA)、肝纤四项的含量。结果与模型组比较,金钗石斛多糖中、大剂量能显著降低大鼠血清中ALT、AST、MDA、肝纤四项水平(P<0.05),提高SOD的表达水平(P<0.05),各阳性药物干预组肝纤维化程度均有所减轻(P<0.05)。结论金钗石斛多糖对肝纤维化大鼠肝功能损伤具有明显治疗作用,其机制与石斛多糖提高大鼠抗氧化能力,减轻肝脏炎症反应有关。
Objective To study the protective effect of polysaccharides from Dendrobium nobile on hepatic fibrosis induced by carbon tetrachloride and ethanol in rats and its possible mechanism. Methods SD rats were randomly divided into normal group, model group, western medicine group, Chinese patent medicine group, low dose of Dendrobium polysaccharide group, middle dose of Dendrobium polysaccharide group and high dose group of Dendrobium officinale polysaccharide. Other groups except normal group were treated with carbon tetrachloride Ethanol-induced hepatic fibrosis model in rats, after 8 weeks of model making success, were given the appropriate drug intervention, once a day for 4 weeks, the normal group, the model group given saline. The degree of hepatic fibrosis was observed by HE staining. The contents of alanine aminotransferase (ALT), aspartate aminotransferase (AST), superoxide dismutase (SOD), malondialdehyde (MDA) ), Liver fiber four content. Results Compared with the model group, high dose of polysaccharides from Dendrobium nobile could significantly reduce the serum levels of ALT, AST, MDA and liver fibrosis (P <0.05), and increase the expression of SOD (P <0.05) Positive drug intervention group liver fibrosis levels were reduced (P <0.05). Conclusion Dendrobium nobile polysaccharides have a significant therapeutic effect on hepatic injury induced by hepatic fibrosis in rats. The mechanism of the effect is that Dendrobii polysaccharides can improve the antioxidant capacity and reduce the inflammatory reaction in the liver.