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本实验以分属特早熟、中熟和晚熟三类不同抗冷好别的６个棉花品种为材料，将其幼苗经４℃低温处理７小时，测定非蛋白氮的含量，并分析其变化情况。结果表明：所试６个品种的幼苗，经过低温锻炼后，其非蛋白氮含量都有不同程度的提高。提高的幅度虽因品种不同而异，但同一抗冷级别的不同品种的非蛋白氦的提高率与抗冷性成明显的正相关。因此，可以将低温处理后的非蛋白氮的提高率作为划分棉花苗期抗冷级别的生化指标之一。在实验事实的基础上，本文从理论上对低温胁迫下，非蛋白氮积累的可能途径以及抗冷机理进行了较为详细的探讨。
In this experiment, we divided the six cotton cultivars, which were special precocious, medium maturity and late maturity, and were treated with low temperature for 4 hours at 4 ℃ to determine the content of non-protein nitrogen and analyze the changes . The results showed that the seedlings of the six cultivars tested improved their non-protein nitrogen content to some extent after low temperature exercise. Although the range of increase depends on the variety, the improvement rate of non-protein helium and the cold resistance of different varieties of the same cold resistance level are obviously positively correlated. Therefore, the improvement rate of non-protein nitrogen after low temperature treatment can be used as one of the biochemical indexes to classify the cold resistance level of cotton seedling. Based on the experimental facts, this paper theoretically discussed the possible ways of non-protein nitrogen accumulation and the mechanism of cold resistance under low temperature stress.