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Objective: The aim of our study was to investigate the effect of Cinobufacini injection on the proliferation and apoptosis of human hepatocarcinoma HepG-2 cells.Methods: Cells proliferation was assessed by MTT assay,cells morphologic was observed by the inverted microscopy,Annexin V/PI stain was used to detect the apoptosis and necrosis of the tumor cells.The expression of TOPO I mRNA and TOPO II mRNA were examined by RT-PCR.Results: Cinobufacini injection significantly inhibited HepG-2 cells proliferation in doseand time-dependent ways.After Cinobufacini injection intervention,HepG-2 cells showed typical morphological changes: cells changed from polygon into round,chromatin looseness and karyolysis were observed.The percentages of apoptosis were 88.49%,76.02%,61.73% corresponding to the 48 h interference of 0.42 μg/mL,0.21 μg/mL,0.105 μg/mL Cinobufacini injection,perspectively.RT-PCR assay showed that Cinobufacini injection down-regulated TOPO I and TOPO II expression at mRNA level.Conclusion: Cinobufacini can inhibit human hepatocarcinoma HepG-2 cells growth and induce tumor cells apoptosis,the mechanism of which might partly relate to the down-regulation of TOPO I mRNA and TOPO II mRNA induced by Cinobufacini injection.
Objective: The aim of our study was to investigate the effect of Cinobufacini injection on the proliferation and apoptosis of human hepatocarcinoma HepG-2 cells. Methods: Cells proliferation was assessed by MTT assay, cells morphologic was observed by the inverted microscopy, Annexin V / PI stain was used to detect the apoptosis and necrosis of the tumor cells. The expression of TOPO I mRNA and TOPO II mRNA were examined by RT-PCR. Results: Cinobufacini injection significantly inhibited HepG-2 cells proliferation in dose and time-dependent ways. After Cinobufacini injection intervention, HepG-2 cells showed typical morphological changes: cells changed from polygon into round, chromatin looseness and karyolysis were observed. 88.49%, 76.02%, 61.73% corresponding to the 48 h interference of 0.42 μg / mL, 0.21 μg / mL, 0.105 μg / mL Cinobufacini injection, perspectively. RT-PCR assay showed that Cinobufacini injection down-regulated TOPO I and TOPO II expression at mRNA level .Conclusion: Cinobufacini can inhibit human hepatocarcinoma HepG-2 cell growth and induce tumor cell apoptosis, the mechanism of which might partly relate to the down-regulation of TOPO I mRNA and TOPO II mRNA induced by Cinobufacini injection.