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目的调查云南省贡山县鼠疫指示动物血清F1抗体情况,为该地是否存在鼠疫自然疫源地提供依据。方法 2014年10-11月在贡山县的3个乡镇6个自然村19个村组采集鼠疫指示动物血清,采用间接血凝试验(IHA)、酶联免疫吸附试验(ELISA)、胶体金免疫层析(GICA)3种方法进行鼠疫F1抗体的实验室检测。结果共检测指示动物血清293份,其中犬血清288份、猫血清5份。IHA检出阳性血清8份,阳性率为2.73%(8/293),ELISA、GICA结果一致,分别检出阳性血清11份,阳性率为3.75%(11/293)。阳性血清均为犬血清。3种方法进行比较,ELISA阳性率优于IHA(χ~2=7.70,P<0.01),与GICA无差异。结论贡山县鼠疫指示动物血清学检测阳性,需引起重视并作进一步调查研究。
Objective To investigate the situation of F1 antibody in serum of plague-indicating animals in Gongshan County, Yunnan Province, and provide the basis for the existence of plague natural foci. Methods Serum of the plague-indicating animals was collected from 19 villages in 6 natural villages of 3 townships in Gongshan County from October to November in 2014. The serum levels of IL-1 and IL-1 were detected by indirect hemagglutination test (IHA), enzyme-linked immunosorbent assay (ELISA) Analysis of (GICA) 3 methods for the detection of plague F1 antibodies in the laboratory. Results A total of 293 animal serum samples were detected, of which 288 were dog serum and 5 cat serum. The results of ELISA and GICA showed that 11 positive sera were detected by ELISA, the positive rate was 2.73% (8/293), and 11 positive sera were detected respectively. The positive rate was 3.75% (11/293). Positive sera are dog serum. The positive rate of ELISA was better than IHA (χ ~ 2 = 7.70, P <0.01), but no difference with GICA. Conclusion The serological detection of plague in Gongshan County indicates that it should be taken seriously and further investigated.