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由染色体断片形成的微核比山整条染色体形成的微核小。这一点已由Yamamoto 等人于1980年在小鼠骨髓多染性红细胞的微核研究中得到证实。然而,人淋巴细胞染色体的长短相差悬殊,断片和染色体交错分布,且都能形成微核。本研究的日的是探讨Yamamoto 等人的发现能否适用于人淋巴细胞微核。方法:取健康人静脉血于含肝素的试管内。培养前将含有15%胎牛血清、PHA 和白细胞的RPMI-1640培养基用X 射线(200rad)、丝裂霉素C(0.33μg/ml)或用乙酰甲基秋水仙素(0.6μg/ml)处理,并在37℃恒温下培养。长春新碱(0.01μg/ml)于开始培
Micronuclei formed by chromosome fragments are smaller than micronuclei formed by chromosomes throughout the mountain. This was confirmed by Yamamoto et al. in 1980 in a micronucleus study of mouse bone marrow multistaining red blood cells. However, the length of chromosomes in human lymphocytes is very different, fragments and chromosomes are staggered, and all of them can form micronuclei. The date of this study was to investigate whether Yamamoto et al.’s findings can be applied to human lymphocyte micronuclei. Methods: Take blood from healthy people in heparin-containing tubes. RPMI-1640 medium containing 15% fetal bovine serum, PHA, and white blood cells was pre-cultured with X-ray (200 rad), mitomycin C (0.33 μg/ml) or Acetyl colchicine (0.6 μg/ml) ) Treat and incubate at a constant temperature of 37°C. Vincristine (0.01μg/ml) started