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银纳米粒子有强烈的等离子体共振散射特性,可作为光散射光谱探针用于示踪和可视化分析.通过研究固载于载玻片上的蛋白质对银纳米粒子的吸附,发现吸附在载玻片上的银纳米粒子在普通的白光发光二极管(LED)照射下,肉眼就能看见其散射光.据此建立可视化检测蛋白质在载玻片上最小完全固载量的方法.
Silver nanoparticles have strong plasmonic resonance scattering properties and can be used as light scattering spectroscopy probes for tracing and visualization analysis.By studying the adsorption of silver nanoparticles immobilized on glass slides, we find that adsorption on glass slides Of silver nanoparticles can be seen by the naked eye under normal white light emitting diode (LED) illumination, thereby establishing a method for visualizing the minimum and complete protein loading on glass slides.