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目的:寒、热证候大鼠大肠癌启动时ATP酶和SDH酶活性的变化。方法:将156只大鼠随机分为寒模组、热模组和空白组。寒模组和热模组分别制作寒证、热证模型。第6周开始于大鼠颈背部注射DMH制作大肠癌模型,同时从寒模组中随机取一半大鼠额外给与反左金丸汤剂灌胃,作为寒模反左金丸组;从热模组中随机取一半大鼠额外给与左金丸汤剂灌胃,作为热模左金丸组。期间定期分批处死大鼠,取肠组织进行HE染色,检测肠组织ATP酶活性、SDH酶活性。结果:大鼠在注射DMH 12周及持续每日冷、热刺激后,寒模组与热模组的SDH和ATP酶活性皆出现先升高再降低的过程,而寒模反左金丸组和热模左金丸组相对平稳,维持在较低水平。结论:寒证模型和热证模型大鼠在大肠癌启动时皆会出现阶段性热证,考虑与肿瘤启动时的炎症发热有关,而对应给与的左金丸和反左金丸对阶段性热证有抑制作用。
Objective: To investigate the changes of ATPase and SDH activities of colorectal cancer in rats with cold and heat syndrome. Methods: 156 rats were randomly divided into cold model group, hot model group and blank group. Cold modules and hot modules were produced cold syndrome, heat syndrome model. From the 6th week, DMH was injected into the neck of the neck to make the model of colorectal cancer. At the same time, half of the rats in the cold model group were given anti-Zuo Jintang decoction orally, Half of rats were randomly selected to give Zuo Jinwan decoction gavage, as the hot die Zuojin pill group. During the period of rats were killed in batches on a regular basis, take intestinal tissue HE staining, detection of intestinal ATPase activity, SDH enzyme activity. Results: The SDH and ATPase activities of cold and hot models both increased and then decreased at 12 weeks after DMH injection and sustained daily cold and heat stimulation, while the cold mode anti-Zuojin pill group Hot die Zuojin pill group is relatively stable, maintained at a low level. Conclusion: Cold syndrome model and heat syndrome model rats in the start of colorectal cancer will appear phased heat syndrome, consider the inflammation and fever at the time of tumor initiation, and the corresponding left Zuo Jin Wan and anti-Zuo Jin Wan on the stage of heat syndrome Inhibit effect.