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目的核苷酸切除修复交叉互补基因2/人类着色性干皮病基因D(excision repair cross complementation group2/Dxeroderma pigmentosum group D,ERCC2/XPD)单核苷酸多态(single nucleotide polymorphism,SNP)与肺癌发病相关联。本研究通过对近15年间发表的相关文献进行Meta分析,评价ERCC2/XPD Asp312Asn位点SNP与肺癌发病的关联。方法检索2000-01-2014-05年PubMed、中国知网(CNKI)、维普资讯网(cqVIP)和万方全文数据库中关于ERCC2/XPD基因312位点SNP与肺癌发病关联的病例对照研究。按拟定的入选标准和排除标准进行筛选,提取符合纳入标准文献的相关数据,采用RevMan 4.2软件计算合并相对危险度(OR值)及其95%可信区间(95%CI),同时绘制漏斗图,估计发表偏倚的影响。结果共纳入国内外文献20篇,累计病例组7 525例,对照组8 570例。Asp/Asn versus Asp/Asp异质性检验结果显示,χ~2=19.97,P=0.40。采用固定效应模型分析,合并OR=1.02,95%CI为0.94~1.10,P=0.65。Asn/Asn versus Asp/Asp异质性检验结果显示,χ~2=20.99,P=0.28。采用固定效应模型分析,合并OR=1.22,95%CI为1.07~1.40,P=0.004。根据种族分组后,结果显示,无论在高加索人群,还是亚洲人群中携带Asn/Asn基因型个体与肺癌发病升高均有关,差异有统计学意义,P<0.05。结论 ERCC2/XPD基因312位点多态与肺癌发病风险相关联,携带312位点Asn/Asn基因型的个体,其肺癌发病风险显著高于携带Asp/Asp基因型的个体,按种族进行分层分析后,此关联仍然存在。
Objective Nucleotide Excision Repair Cross Complementation group 2 / Dxeroderma pigmentosum group D (ERCC2 / XPD) Single nucleotide polymorphisms (SNPs) and lung cancer Associated with the disease. In this study, meta-analysis of relevant literature published in the past 15 years to evaluate the relationship between SNP in ERCC2 / XPD Asp312Asn site and lung cancer. Methods A case-control study was performed on the association of SNP at SNP 312 locus with ERCC2 / XPD gene in lung cancer from PubMed, CNKI, cqVIP and Wanfang full-text database from 2000-01 to 2014-05. According to the proposed inclusion criteria and exclusion criteria, relevant data were extracted and included in the standard literature, RevMan 4.2 software was used to calculate the combined relative risk (OR) and 95% confidence interval (95% CI) , Estimate the impact of publication bias. Results Totally 20 domestic and foreign literatures were included, including 7 525 cases in total and 8 570 cases in control group. Asp / Asn versus Asp / Asp heterogeneity test showed that χ ~ 2 = 19.97, P = 0.40. Fixed-effects model analysis, with OR = 1.02, 95% CI 0.94 to 1.10, P = 0.65. Asn / Asn versus Asp / Asp heterogeneity test showed that χ ~ 2 = 20.99, P = 0.28. Using the fixed-effects model analysis, OR = 1.22 with a 95% CI of 1.07-1.40, P = 0.004. According to ethnic grouping, the results showed that individuals with Asn / Asn genotypes in Caucasians and Asians were associated with an increased incidence of lung cancer with a statistically significant difference (P <0.05). CONCLUSION: The polymorphism of the 312 locus in ERCC2 / XPD gene is associated with the risk of lung cancer. The individuals with the 312 Asn / Asn genotype have a significantly higher risk of developing lung cancer than those with the Asp / Asp genotype and are stratified by race After the analysis, this association still exists.