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作者指出,通过测定正常和异常肾上腺功能的儿童循环硫酸孕烯醇酮(PS),有助于对婴幼儿肾上腺机能障碍的诊断。 材料和方法:将血浆稀释至1/20～1/2000(根据其年龄或病理情况)测定PS。每份血浆以累积方式分成3份(100～400μl);以200μl抗体加进100μl的~3H-孕烯醇酮进行试管培养。在4℃培养一昼夜或在37℃培养90分钟,后用右旋糖酐-活性炭溶液分离。用Cis Sorin药箱测ACTH和皮质醇;用RIA法测17α孕酮;用竞争结合方法测11-脱氧皮质醇(S)。 受检者及项目:肾上腺功能检查包括测定其基础水平与被激发后的变化。①短时ACTH试验:被测者12例(7个月～12岁),静注含造影剂的合成ACTH(1mg/m~2体表面积),在注射前及注射后515、60分钟分别测PS、皮质醇;②长时ACTH刺激试验;受检者66名(出生2日～15岁),肌注ACTH,每12小时1次,连用3日,③用甲吡酮试
The authors point out that the diagnosis of adrenal dysfunction in infants and young children can be facilitated by circulating circulating progesterone sulfate (PS) in children with normal and abnormal adrenal function. Materials and Methods: Plasma was diluted to 1/20 to 1/2000 (depending on age or pathology) to determine PS. Each plasma was divided into 3 parts cumulatively (100-400 μl); test tubes were incubated with 200 μl of antibody plus 100 μl of ~ 3H-pregnenolone. Incubate at 4 ° C for one day and night or at 37 ° C for 90 minutes and then dextran-activated carbon solution. ACTH and cortisol were measured using a Cis Sorin kit; 17α progesterone was assayed by RIA; and 11-deoxycortisol (S) was measured by competitive binding assay. Subjects and programs: Adrenal function tests include measuring the basal level and the changes after the challenge. ① Short-term ACTH test: 12 cases (7 months to 12 years old) of the test subjects were intravenously injected with synthetic ACTH (1 mg / m 2 body surface area) containing contrast medium, before injection and at 515 and 60 minutes after injection PS, cortisol; ② long-term ACTH stimulation test; 66 subjects (born 2 to 15 years old), intramuscular ACTH, once every 12 hours, once every 3 days, with metyrapone test