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目的：研究三乙酰莽草酸（ＴＳＡ）对血小板聚集功能的抑制作用及其作用机理。方法：用比浊法测定血小板聚集功能，分光光度法测定ＭＤＡ的含量，放免法测定ＴＸＢ２，６酮ＰＧＦ１α，ｃＡＭＰ和ｃＧＭＰ的含量。结果：ＴＳＡ１２５，２５，５０，１００和２００ｍｇ·ｋｇ－１ｉｇ明显抑制ＡＤＰ和胶原诱导的大鼠血小板聚集；ＴＳＡ１２５，５０和２００ｍｇ·ｋｇ－１ｉｇ显著增加大鼠血小板内ｃＡＭＰ水平，但不影响ｃＧＭＰ水平。ＴＳＡ２００ｍｇ·ｋｇ－１对ＡＡ诱导的血小板中ＭＤＡ的生成，ＡＤＰ诱导的血小板中ＴＸＢ２和腹主动脉壁６酮ＰＧＦ１α的生成有轻度抑制作用。结论：ＴＳＡ抑制血小板聚集作用部分与血小板内ｃＡＭＰ水平升高有关。
Objective: To study the inhibitory effect and its mechanism of triacetylshikimic acid (TSA) on platelet aggregation. Methods: Platelet aggregation was measured by turbidimetric method. MDA content was determined by spectrophotometry. The levels of TXB2, 6-keto-PGF1α, cAMP and cGMP were determined by radioimmunoassay. Results: TSA12.5, 25, 50, 100 and 200 mg · kg-1ig significantly inhibited platelet aggregation induced by ADP and collagen in rats; TSA12.5, 50 and 200 mg · kg-1ig significantly increased platelet cAMP levels in rats Does not affect cGMP levels. TSA200mg · kg-1 on AA-induced platelet MDA, ADP-induced platelet TXB2 and abdominal aortic wall 6 ketone PGF1α production was slightly inhibited. CONCLUSIONS: TSA inhibits platelet aggregation in part by an increase in platelet cAMP levels.