论文部分内容阅读
目的:探讨核受体PXR在结肠癌细胞增殖和化疗敏感性中的作用。方法:分别用RT-PCR和westernblot方法检测PXR在人结肠癌细胞株LS174T、LOVO、HT29、HCT116中的表达情况。通过质粒稳定转染方法建立PXR敲低的细胞株。用MTT方法分析利福平活化PXR或稳定转染敲低PXR后,细胞增殖和化疗敏感性的改变。结果:在结肠癌细胞株LS174T、LOVO、HT29、HCT116中,LS174T细胞的PXR表达水平最高。利福平处理后,LS174T细胞中PXR表达增强。利福平活化PXR或稳定转染敲低PXR后,相应地促进或抑制细胞增殖,降低或提高细胞对化疗药物的敏感性。结论:PXR能促进结肠癌细胞增殖,提高细胞对化疗药物的敏感性,可能在结肠癌多药耐药机制中具有重要作用。
Objective: To investigate the role of nuclear receptor PXR in the proliferation and chemosensitivity of human colon cancer cells. Methods: The expression of PXR in human colorectal cancer cell lines LS174T, LOVO, HT29 and HCT116 were detected by RT-PCR and western blot respectively. PXR knockdown cell lines were established by plasmid stable transfection methods. MTT assay was used to analyze the changes of cell proliferation and chemosensitivity after rifampin-activated PXR or stable transfection knockdown of PXR. Results: LS174T cells had the highest PXR expression in colon cancer cell lines LS174T, LOVO, HT29 and HCT116. After rifampicin treatment, PXR expression was enhanced in LS174T cells. Rifampicin activates or inhibits the proliferation of PXRs after activation of PXR or stable transfection knockdown of PXR, which reduces or increases the sensitivity of cells to chemotherapeutic drugs. Conclusion: PXR can promote the proliferation of colon cancer cells and increase the sensitivity of cells to chemotherapeutic drugs, which may play an important role in the multidrug resistance of colon cancer.