利用酚试剂研究了漆酶活性的比色测定方法。在实验条件下焦性没食子酸氧化产物的吸光值倒数与反应时间的倒数成正比,符合公式1/Q=(b/a)(1/t)+1/a。于是可用上式中a/b来表征漆酶的催化活性。实测数据表明,本法比常规比色法和通气比色法灵敏。 The phenol reagent was used to study the colorimetric determination of laccase activity. Under the experimental conditions, the reciprocal of the absorbance of the pyrogallol oxidized product is proportional to the reciprocal of the reaction time, which is in accordance with the formula 1 / Q = (b / a) (1 / t) + 1 / a. Then the above formula a / b can be used to characterize the catalytic activity of laccase. The measured data show that this method is more sensitive than conventional colorimetry and ventilation colorimetry.