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目的:探讨雄黄中砷对大鼠脑组织氨基酸类神经递质的影响。方法:将32只Wistar大鼠随机分为4组,每组8只,以0.5%CMC-Na水溶液为混悬介质,实验组(0.3、0.9、2.7 g/kg)灌胃给予雄黄,对照组给予等体积的0.5%CMC-Na,连续灌胃给予雄黄混悬液2 w。采用氢化物发生原子吸收法测定脑组织中砷含量,高效液相色谱法测定脑组织中8种氨基酸类神经递质(天冬氨酸、谷氨酸、同型半胱氨酸、谷氨酰胺、丝氨酸、甘氨酸、γ-氨基丁酸、牛磺酸)含量。结果:随着灌胃给予雄黄剂量的增加,大鼠脑组织中砷含量逐渐增加,说明雄黄中的砷可通过血脑屏障在脑组织蓄积。给予雄黄2 w后,与对照组比较,低剂量雄黄染毒组大鼠脑组织中丝氨酸、甘氨酸和γ-氨基丁酸含量明显增加。中、高剂量雄黄染毒组大鼠脑组织中同型半胱氨酸、谷氨酰胺、丝氨酸和天冬氨酸含量明显低于对照组。结论:雄黄中砷可在脑组织蓄积,对大鼠脑组织氨基酸类神经递质产生影响,氨基酸类神经递质可能是雄黄毒性作用的靶点之一。
Objective: To investigate the effect of realgar on the amino acid neurotransmitters in rat brain. Methods: Thirty-two Wistar rats were randomly divided into 4 groups with 8 rats in each group. 0.5% CMC-Na aqueous solution was used as the suspending medium. The experimental group (0.3, 0.9 and 2.7 g / kg) An equal volume of 0.5% CMC-Na was administered, followed by continuous oral administration of realgar suspension for 2 weeks. The contents of arsenic in brain tissue were determined by hydride generation atomic absorption spectrometry. The contents of 8 amino acid neurotransmitters (aspartic acid, glutamic acid, homocysteine, glutamine, Serine, glycine, gamma-aminobutyric acid, taurine) content. Results: As the dose of realgar increased, the content of arsenic in rat brain gradually increased, indicating that arsenic in realgar could accumulate in the brain through the blood-brain barrier. Compared with the control group, the content of serine, glycine and γ-aminobutyric acid in the brain tissue of low-dose realgar-treated rats significantly increased after giving realgar for 2 weeks. The contents of homocysteine, glutamine, serine and aspartic acid in brain of middle-dose and high-dose realgar-treated rats were significantly lower than those of the control group. Conclusion: Arsenic in realgar can accumulate in the brain tissue and affect amino acid neurotransmitters in brain tissue of rats. Amino acid neurotransmitters may be one of the targets of virulence of realgar.