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为了探讨凋亡相关基因ｂｃｌ－２、ｂａｘ及Ｆａｓ在乙肝病毒相关性肝硬化形成过程中的作用。应用免疫组化方法观察３０例肝硬化组织中凋亡相关基因ｂｃｌ－２、ｂａｘ及Ｆａｓ表达及ＨＢｓＡｇ的阳性率。２１例ＨＢｓＡｇ阳性的肝硬化组织中，４例ｂｃｌ－２阳性，阳性率为１９．１％，１６例ｂａｘ阳性，阳性率为７６．２％，１５例Ｆａｓ阳性，阳性率为７１．４％。９例ＨＢｓＡｇ阴性的肝硬变中１例ｂｃｌ－２阳性，阳性率为１１．１％，ｂａｘ有４例阳性，阳性率为４４．４％，Ｆａｓ有２例阳性，阳性率为２２．２％。伴与不伴乙肝病毒感染的两组肝硬化中ｂｃｌ－２表达无明显差异（Ｐ＞０．０５），而ｂａｘ和Ｆａｓ差异有显著性（Ｐ＜０．０５）。提示ｂａｘ和Ｆａｓ可能参与了乙肝病毒致肝细胞的损伤过程。
To investigate the role of apoptosis-related genes bcl-2, bax and Fas in the development of hepatitis B virus-related cirrhosis. Immunohistochemistry was used to observe the expression of bcl-2, bax, Fas and the positive rate of HBsAg in 30 cirrhotic patients. Of 21 HBsAg positive cirrhotic tissues, 4 were bcl-2 positive, the positive rate was 19.1%, 16 were bax positive, the positive rate was 76.2%, 15 were Fas positive, the positive rate was 71.4% . In 9 cases of HBsAg-negative liver cirrhosis, 1 case of bcl-2 positive, the positive rate was 11.1%, 4 cases of bax positive, the positive rate was 44.4%, Fas 2 cases were positive, the positive rate was 22.2 %. There was no significant difference in bcl-2 expression between the two groups with and without HBV infection (P> 0.05), while there was a significant difference between bax and Fas (P <0.05). Tip bax and Fas may be involved in hepatitis B virus induced liver injury process.