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Prunus necrotic ring spot virus (PNRSV) and grapevine fanleaf virus (GFLV) were detected by fluoroimmunoassay usingbacterial magnetic particles (BMPs), and a double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA).For the fluoroimmunoassay, fluorescein isothiocyanate labeled anti-PNRSV antibody or anti-GFLV antibody was conjugatedonto BMPs of Magnetospirillum gryphiswaldense MSR-1. With this method, a very low minimum antigen concentration(1 × 106 dilution of the original sample concentration) could be detected. Using DAS-ELISA, the minimum antigen detectionconcentration was the original sample concentration. Thus, comparing these two methods, a BMP-based method couldincrease the sensitivity up to six orders of magnitude (106) higher than an ELISA-based method of detection PNRSV andGFLV.
For the fluoroimmunoassay, fluorescein isothiocyanate labeled anti- (FACS) was detected by fluoroimmunoassay using bacterium magnetic particles (BMPs), and a double antibody sandwich enzyme linked immunosorbent assay PNaseV ELISA or the anti-GFLV antibody was conjugatedonto BMPs of Magnetospirillum gryphis waldense MSR-1. With this method, a very low minimum antigen concentration (1 × 10 6 dilution of the original sample concentration) could be detected. detectionconcentration was the original sample concentration of the BMP-based method couldincrease the sensitivity up to six orders of magnitude (106) higher than an ELISA-based method of detection of PNRSV andGFLV.