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目的研究束缚-浸水应激(RWIS)大鼠疑核(NA)中胆碱能、儿茶酚胺能神经元的活动情况。方法随机将10只雄性Wistar大鼠分为对照组和应激组(RWIS组),采用Fos、胆碱乙酰转移酶(ChAT)或酪氨酸羟化酶(TH)免疫组织化学双标技术,统计对照组和应激组Fos、ChAT或TH单标阳性神经元以及Fos+ChATT或Fos+TH双标阳性神经元数目。结果 NA中ChAT免疫阳性神经元胞体呈梭形或多角形,有较多的TH阳性终末。与对照组相比,RWIS组大鼠NA大量神经元表达Fos(P<0.05);RWIS组Fos+ChAT双标阳性神经元数目也较对照组显著增加(P<0.05),占ChAT免疫阳性神经元的比例在对照组和RMS组中分别为7.5%、29.8%(P<0.01)。结论 NA中胆碱能神经元参与RWIS调控过程,而这些神经元可能接受其他核团儿茶酚胺能神经元的纤维投射。
Objective To investigate the activity of cholinergic and catecholaminergic neurons in skeletal-water immersion stress (RWIS) rat suspicious nuclei (NA). Methods Ten male Wistar rats were randomly divided into control group and stress group (RWIS group). Fos, choline acetyltransferase (ChAT) or tyrosine hydroxylase (TH) The numbers of single positive neurons of Fos, ChAT or TH and double positive neurons of Fos + ChATT or Fos + TH were counted in control group and stress group. Results ChAT immunopositive neurons in NA showed fusiform or polygonal cells with more TH-positive terminals. Compared with the control group, the number of neurons in NA of RWIS group was significantly higher than that of the control group (P <0.05), and the number of Fos + ChAT double positive neurons in RWIS group was also significantly increased (P <0.05) The proportion of elements in the control and RMS groups was 7.5% and 29.8%, respectively (P <0.01). Conclusions Cholinergic neurons in NA are involved in the process of RWIS regulation. These neurons may be involved in the fiber projection of catecholaminergic neurons in other nuclei.