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目的探讨在华支睾吸虫感染中,肝脏局部高水平的嗜酸性粒细胞浸润现象是否与内源性白细胞介素33(IL-33)有关。方法建立华支睾吸虫感染小鼠模型,利用流式细胞术检测感染小鼠肝脏嗜酸性粒细胞比例变化;利用抗ST2抗体阻断IL-33与ST2的作用,观察对感染后小鼠肝脏局部嗜酸性粒细胞浸润程度的影响。结果华支睾吸虫感染后,肝脏嗜酸性粒细胞比率显著增多。阻断IL-33与ST2的作用,能显著降低感染小鼠肝脏局部嗜酸性粒细胞比例(12.07%±1.58%vs 7.69%±0.68%;P<0.01)。结论华支睾吸虫感染后,IL-33参与肝脏局部高水平嗜酸性粒细胞的维持。
Objective To investigate whether high level of eosinophil infiltration in the liver is associated with endogenous interleukin-33 (IL-33) in Clonorchis sinensis infection. Methods The mouse model of Clonorchis sinensis infection was established. The percentage of eosinophils in infected mice was detected by flow cytometry. The anti-ST2 antibody was used to block the effect of IL-33 and ST2. Eosinophil infiltration degree of influence. Results Clonorchis sinensis infection, liver eosinophil ratio was significantly increased. Blocking the effect of IL-33 and ST2 significantly reduced the local eosinophil ratio in infected mice (12.07% ± 1.58% vs 7.69% ± 0.68%; P <0.01). Conclusion After inoculation of Clonorchis sinensis, IL-33 is involved in the maintenance of local high level of eosinophils in the liver.