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目的:探讨小檗碱对小鼠胚胎间充质干细胞C3H10t_(1/2)细胞增殖和成脂分化的影响。方法:采用MTS法对细胞增殖情况进行检测,同时通过油红O染色,异丙醇溶解法检测细胞成脂分化情况,GPO-PAP酶法检测细胞中甘油三酯(TG)含量,RT-PCR检测成脂分化关键转录因子CCAAT增强子结合蛋白α(C/EBPα)、过氧化物酶体增殖物激活受体γ(PPARγ)和分化标志物脂肪型脂肪酸结合蛋白(fatty acid binding protein,FABP4)mRNA的表达。结果:小檗碱浓度在50μmol/L以下,可促进C3H10t_(1/2)细胞的增殖;当小檗碱浓度达到10μmol/L时可显著抑制C3H10t_(1/2)细胞成脂分化及甘油三酯的堆积,并呈一定的剂量依赖性;10μmol/L小檗碱处理组C3H10t_(1/2)细胞PPARγmRNA表达在诱导分化第4、7、10天显著降低(P<0.05或P<0.01),C/EBPαmRNA的表达在分化第1、7、10天显著降低(P<0.05或P<0.01),FABP4 mRNA的表达在分化第10天显著降低(P<0.05)。结论:小檗碱能够促进C3H10t_(1/2)细胞增殖,并能够抑制其成脂分化。
Objective: To investigate the effects of berberine on the proliferation and adipogenic differentiation of mouse embryonic mesenchymal stem cells C3H10t_ (1/2). Methods: The proliferation of cells was detected by MTS method. The differentiation of cells was detected by oil red O staining and isopropanol dissolution method. The content of triglyceride (TG) was detected by GPO-PAP method, (C / EBPα), peroxisome proliferator-activated receptor γ (PPARγ) and the marker of fatty acid binding protein (FABP4), the key transcriptional factors of adipogenic differentiation, mRNA expression. Results: The concentration of berberine below 50μmol / L could promote the proliferation of C3H10t_ (1/2) cells. Berberine could inhibit the adipogenic differentiation of C3H10t_ (1/2) cells and triglyceride (P <0.05 or P <0.01) on the 4th, 7th and 10th day after differentiation. The PPARγ mRNA expression of C3H10t_ (1/2) cells treated with 10μmol / L berberine was significantly decreased (P <0.05 or P <0.01). The expression of FABP4 mRNA was significantly decreased on the 10th day after differentiation (P <0.05). Conclusion: Berberine can promote the proliferation of C3H10t_ (1/2) cells and inhibit its adipogenic differentiation.