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目的：通过体外实验评价紫杉醇对胶质母细胞瘤的放射增敏作用。方法：应用人胶质母细胞瘤细胞系ＢＴ３２５。（１）细胞暴露于不同浓度紫杉醇，分别于６、１２、２４、４８、７２小时收集标本、作流式细胞术细胞周期分析。（２）细胞经紫杉醇和（或）照射处理后，绘制细胞生长曲线，计算出１０％存活率时的放射增敏比（ＳＥＲ）。结果：细胞暴露于紫杉醇后发生了Ｇ２－Ｍ期阻滞，当紫杉醇作用时间较长或浓度较高时，Ｇ２－Ｍ期细胞的比率也较高，紫杉醇＋照射组的细胞生长受到显著抑制；１、１０、１００ｎｍｏｌ紫杉醇在１０％存活率时的ＳＥＲ分别为１．１、１．９、３．０。结论：紫杉醇能将细胞阻滞在对射线最敏感的Ｇ２－Ｍ期；紫杉醇能增强胶质母细胞瘤的放射生物学效应，值得进一步研究
Objective: To assess the radiosensitization of paclitaxel on glioblastoma by in vitro experiments. Methods: Human glioblastoma cell line BT325 was used. (1) The cells were exposed to different concentrations of paclitaxel and collected at 6, 12, 24, 48, and 72 hours for flow cytometric cell cycle analysis. (2) After the cells were treated with paclitaxel and/or irradiation, the cell growth curve was plotted, and the radiosensitivity ratio (SER) at the 10% survival rate was calculated. RESULTS: G2-M phase arrest occurred after exposure to paclitaxel. When paclitaxel had a longer duration or higher concentration, the ratio of cells in G2-M phase was also higher, and cell growth in paclitaxel plus irradiation group was significantly inhibited. The SERs at 1, 10, and 100 nmol paclitaxel at the 10% survival rate were 1.1, 1.9, and 3.0, respectively. CONCLUSION: Paclitaxel can block cells in the G2-M phase most sensitive to radiation; paclitaxel can enhance the radiobiological effects of glioblastoma and deserve further study.