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目的探明PCR克隆性基因重排检测（基因诊断）在NHL诊断中的意义。方法应用病理组织学、免疫组化、基因诊断方法三结合方案分析178例与会诊31例NHL诊断的作用。结果分析结果显示：以病理组织学为主，辅以免疫组化确诊NHL114例。疑难、早期、微量标本共64例与会诊31例均作了基因诊断。总的阳性率为61．4％（58／95）。其中IgH阳性13例（22．4％），TCRβ芝邱阳性41例（70．6％），双克隆性4例（7％）。本室基因诊断送检率为35．8％（64／178），总确诊率为93．7％（60／64）。结论本文分析证明基因诊断是NHL三结合诊断中确诊疑难病例及分型的最有效的方法。
Objective To explore the significance of PCR cloning gene rearrangement detection (genetic diagnosis) in the diagnosis of NHL. Methods The diagnosis of NHL was performed in 178 patients and 31 patients diagnosed by clinic using histopathological, immunohistochemical and genetic diagnosis methods. The analysis of the results showed that 114 cases of NHL were diagnosed mainly by histopathology and assisted by immunohistochemistry. A total of 64 cases of difficult, early, and microscopic specimens and 31 cases of consultations were genetically diagnosed. The overall positive rate was 61.4% (58/95). Of these, 13 (22.4%) were positive for IgH, 41 (70.6%) were positive for TCRβ, and 4 (7%) were double-clone. The rate of genetic diagnosis in this laboratory was 35.8% (64/178), and the total diagnosis rate was 93.7% (60/64). Conclusions This analysis demonstrates that genetic diagnosis is the most effective method for diagnosing difficult cases and typing in the NHL triple binding diagnosis.