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目的 应用原子力显微镜(AFM)对电磁脉冲(EMP)辐射前、后培养的垂体细胞膜进行观察,以探讨EMP对其细胞膜影响的直接证据。方法 对Wistar大鼠垂体细胞在6孔板中进行原代培养,在培养第 4天时,用高场强 EMP模拟源(场强为 6×10~4V/m,脉冲上升时间为 20 ns,脉宽为 30 ms),以 2.5次/min,照射2 min。并于照射后即刻固定细胞,应用日本岛津(SHIMADZU)公司的SPM-9500J3型原子力显微镜对细胞表面进行接触式连续扫描。结果 EMP辐射后即刻就可引垂体细胞膜表面大小不一、类圆形和不规则形的穿孔,穿孔最大口径和深度可达291nm×372nm×11.72nm。结论 EMP辐射后可直接导致垂体细胞膜的穿孔,提示垂体细胞膜可能是EMP生物效应的靶部位之一。
Objective To investigate the direct evidence of the effect of EMP on the cell membrane by atomic force microscopy (AFM) before and after irradiation with EMP. Methods Pituitary cells from Wistar rats were primarily cultured in 6-well plates. On the 4th day of culture, high-field EMP simulation source (field intensity 6 × 10-4V / m, pulse rise time 20 ns, pulse 30 ms wide) at 2.5 strokes / min for 2 min. The cells were fixed immediately after irradiation. The surface of the cells was continuously and contact-scanned by the SPM-9500J3 atomic force microscope from Shimadzu Corporation. Results EMP radiation immediately after the pituitary cell membrane surface can take the size of different types of round and irregular perforation, the maximum diameter and depth of perforation up to 291nm × 372nm × 11.72nm. Conclusion EMP radiation can directly lead to perforation of pituitary cell membrane, suggesting that pituitary cell membrane may be one of the target sites for the biological effects of EMP.