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目的探讨人类白细胞抗原Ⅱ类(HLA-Ⅱ)基因多态性与晚期肝脾型日本血吸虫病的遗传关联性。方法应用基因芯片分析技术对武汉市蔡甸45例晚期肝脾型日本血吸虫病患者(实验组)和44例慢性日本血吸虫病患者(对照组)的HLA-Ⅱ基因DRB位点等位基因进行基因分型,并比较两组各等位基因频率以及与晚期肝脾型日本血吸虫病的相关性。结果实验组HLA-DRB1*04x等位基因频率明显高于对照组(P<0.01,RR=3.928),而对照组HLA-DRB1*15x等位基因频率明显高于实验组(P<0.01,RR=0.050)。等位基因DRB1*15x总与DRB5*010x/020x连锁,对照组DRB1*15x-DRB5*010x/020x连锁体频率明显高于实验组(P<0.01)。结论HLA-DRB1*04x与晚期肝脾型日本血吸虫病呈正相关,而HLA-DRB1*15x与晚期血吸虫病呈负相关。
Objective To investigate the genetic association between human leukocyte antigen Ⅱ (HLA-Ⅱ) gene polymorphism and advanced hepatosplenic schistosomiasis japonica. Methods The genotypes of HLA-Ⅱ DRB loci in 45 patients with advanced hepatosplenic schistosomiasis japonica (experimental group) and 44 patients with chronic schistosomiasis japonica (control group) were analyzed by gene chip technique. Type, and compared the frequency of each allele and the association with advanced hepatosplenic type Schistosoma japonicum. Results The frequency of HLA-DRB1 * 04x allele in experimental group was significantly higher than that in control group (P <0.01, RR = 3.928), but the frequency of HLA-DRB1 * 15x allele in control group was significantly higher than that in experimental group = 0.050). The allele DRB1 * 15x was always linked with DRB5 * 010x / 020x. The frequency of DRB1 * 15x-DRB5 * 010x / 020x in the control group was significantly higher than that in the experimental group (P <0.01). Conclusion HLA-DRB1 * 04x is positively correlated with advanced liver-spleen type Schistosoma japonicum, while HLA-DRB1 * 15x is negatively correlated with advanced schistosomiasis.