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本文报告由人淋巴结建立一株人淋巴母细胞样细胞系,命名为“昆明总医院淋巴细胞株KGHLY(Kunming General Hospital Lymphoblastoid Cell line KGHLY)。KGHLY细胞悬浮生长,易形成团块,生长旺盛,第30代细胞倍增时间为26h,光镜和电镜下培养细胞的形态和结构与淋巴母细胞样细胞的相似。细胞分泌IgM,表面标志研究显示,无绵羊红细胞受体,这些提示细胞来自B淋巴细胞。核型是非整倍体,染色体众数为44—46条,在体外已维持一年,传代70代,细胞保存液氮复苏良好,此细胞系的建立将是淋巴母细胞样细胞和人一人杂交瘤有用的研究材料。
This article reports the establishment of a human lymphoblastoid cell line from human lymph nodes, named “Kunming General Hospital Lymphoblastoid Cell Line KGHLY.” KGHLY cells grow in suspension and grow easily, The multiplication time of 30th generation cells was 26h, and the morphology and structure of cultured cells were similar to that of lymphoblastoid cells under light and electron microscopy.Immune secretion of IgM, surface marker studies showed that there were no sheep erythrocyte receptors, suggesting that the cells were derived from B lymphocytes . The karyotype is aneuploid, the chromosome number is 44-46, has been maintained in vitro for one year, passage 70 generations, the cell preservation of liquid nitrogen recovery is good, the establishment of this cell line will be lymphoblastoid cells and human one Hybridomas useful research materials.