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目的了解哈尔滨市G9型A组轮状病毒与国内外部分地区G9型毒株VP7基因序列遗传与变异的差异,丰富我国G9型A组轮状病毒VP7基因序列的基础资料。方法通过一步法RT-PCR获得了1株G9型A组轮状病毒VP7蛋白的cDNA片段,对其进行扩增、克隆、测序,用DNASTAR生物软件对其进行序列分析,同时采用参比毒株分组的方法进行序列比对。结果该G9型哈尔滨地方株VP7基因为1 061个碱基,推导326个氨基酸;与中国北京株BJ-CR531进行氨基酸序列比对,一处变异位点在aa221[丝氨酸(Ser)→甘氨酸(Gly)],发生在C区;在分组比对中,哈尔滨G9型毒株所在组的标志性位点在aa8和aa65,均是由丙氨酸(Ala)替代苏氨酸(Thr)。结论哈尔滨G9型毒株所在组的aa8和aa65位点可能成为中国地区A组轮状病毒G9型这一进化分支的关键变异位点。
Objective To understand the genetic variation of VP7 gene of G9 type A rotavirus in Harbin and part of G9 at home and abroad and enrich the basic information of VP7 gene of G9 type A rotavirus in China. Methods A cDNA fragment of VP7 protein of a group A of rotavirus G9 was obtained by one-step RT-PCR. The fragment was amplified, cloned, sequenced and sequenced by DNASTAR software. At the same time, Grouping methods for sequence alignment. Results The amino acid sequence of VP7 gene of Harbin G9 strain was 1061 bp and deduced 326 amino acids. The amino acid sequence of B7 was compared with that of Beijing strain BJ-CR531. One mutation site was found in aa221 [Gly → Gly )] Occurred in region C. In the grouping alignment, the hallmark of Harbin G9 strain was aa8 and aa65, all of which were replaced by alanine (Ala) for threonine (Thr). Conclusion The aa8 and aa65 loci in Harbin G9 strain may be the key mutation sites of the evolutionary branch of G9 type A rotavirus in China.