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以草炭为对照,研究了发酵鸡粪、蚯蚓粪和菇渣作为假单胞菌(Pseudomonas)YT3吸附载体的可行性。结果表明:鸡粪吸水率最低,有效菌体释放效率为93.08%;菇渣的吸水性最高,但其菌体释放效率仅为67.59%;菌体释放效率与载体的阳离子代换量呈极显著负相关。用浸提液培养苜蓿种子36h后,鸡粪、蚯蚓粪和草炭处理的种子发芽指数均在80%以上,初步认定肥料浸提液对植物无毒。随着Pseudomonas YT3接种时间的增加,鸡粪、蚯蚓粪和草炭处理中的活菌数均有一个明显的增加过程;在接种后第30d时,3种有机物料中的最大活菌数分别为8.9×108、7.5×108、5.9×108CFU·g-1,分别为初始接种量的560%、326%和187%;在第180d时,有效活菌数开始小于初始接种值。随着接种时间的延长,菇渣处理的有效活菌数呈一直下降趋势。鸡粪和蚯蚓粪,尤其是鸡粪是适宜的Pseudomonas YT3吸附载体。
Using peat as control, the feasibility of using fermented chicken manure, vermicompost and mushroom residue as the Pseudomonas YT3 adsorption carrier was studied. The results showed that the water absorption rate of chicken manure was the lowest, and the effective bacterial release efficiency was 93.08%. The water absorption of mushroom residue was the highest, but the bacterial release efficiency was only 67.59%. The bacterial cell release efficiency was significantly higher than that of the carrier Negative correlation. After the alfalfa seeds were leached for 36h, the germination indexes of the chicken manure, vermicompost and peat were both above 80%, and the fertilizer leaching solution was initially found to be non-toxic to plants. With the increase of Pseudomonas YT3 inoculation time, the number of viable cells in chicken manure, vermicompost and peat treatment had a significant increase. The maximum viable count of the three organic materials at the 30th day after inoculation was 8.9 × 108,7.5 × 108,5.9 × 108CFU · g-1, which were 560%, 326% and 187% of the initial inoculum size, respectively. At the 180th day, the viable cell number began to be less than the initial inoculum size. With the extension of inoculation time, the effective viable count of mushroom residue treatment has been declining. Chicken manure and vermicompost, especially chicken manure, are suitable Pseudomonas YT3 adsorption carriers.