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近年来,缺血再灌注损伤中氧自由基的作用已被许多直接或间接证据所证实,并得到公认。目前直接测定氧自由基的方法是用电子自旋共振仪(ESR);间接的方法是使用外源性氧自由基清除剂如超氧化物歧化酶(SOD)、过氧化氢酶(CAT)观察其对组织缺血再灌注损伤的保护作用。最近Kinouchi等用携带人SOD 1基因的转基因小鼠(Tg)观察了处于高表达状态的铜锌超氧化物歧化酶(CuZn SOD,其SOD的活力是非转基因小鼠nTg的3.1倍)对小鼠大脑局部缺血再灌注损伤的保护作用,结果发现,缺血24h后,转
In recent years, the role of oxygen free radicals in ischemia-reperfusion injury has been confirmed by many direct and indirect evidences, and has been recognized. At present, direct determination of oxygen free radicals by electron spin resonance (ESR); indirect method is the use of exogenous oxygen free radical scavengers such as superoxide dismutase (SOD), catalase (CAT) observed Its protective effect on tissue ischemia-reperfusion injury. Recently, Kinouchi et al. Used transgenic mice (Tg) carrying human SOD 1 gene to observe the effect of high level of copper-zinc superoxide dismutase (CuZn SOD, SOD activity 3.1 times that of non-transgenic mice) on mice Cerebral ischemia-reperfusion injury protection, the results found that 24h after ischemia, turn