论文部分内容阅读
用小鼠抗早孕实验,观察甲氨蝶呤(MTX)单用和合并用药的抗早孕作用。小鼠于妊娠d7分别一次scMTX8,24和72mg·kg-1可引起胚胎死亡,妊娠d13死亡率分别为180%,555%和603%。给药后测定血清孕酮水平,妊娠d9未发现明显改变,妊娠d13其水平明显降低。小鼠于妊娠d7~9注射MTX24mg·kg-1连续3d引起胚胎全部死亡。hCG和孕酮可拮抗MTX的抗早孕作用。RU486可增强MTX的抗早孕作用,提示两药合用抗早孕有协同作用。
Anti-early pregnancy experiments with mice to observe methotrexate (MTX) single and combined anti-pregnancy effect. Mice in pregnancy d7 once scMTX 8,24 and 72mg · kg-1 can cause embryonic death, pregnancy d13 mortality were 18 0%, 55 5% and 60 3%. Determination of serum progesterone levels after administration, pregnancy d9 found no significant change, pregnancy d13 levels were significantly lower. Mice in pregnancy d7 ~ 9 injection of MTX24mg · kg-1 for three consecutive days caused all embryonic deaths. hCG and progesterone can antagonize the anti-early pregnancy effect of MTX. RU486 can enhance the anti-pregnancy effect of MTX, suggesting a synergistic effect of the two drugs in combination with anti-early pregnancy.