论文部分内容阅读
目的:探讨Twist1调控转录因子Fox M1在上皮性卵巢癌中的表达。方法:采用免疫组织化学SP法检测Fox M1、Twist1在上皮性卵巢癌组织中表达情况,斯皮尔曼秩相关分析其在上皮性卵巢癌组织中的表达相关性;双荧光素酶报告系统检测Twist1对Fox M1的调控作用;Real-time quantitative RT-PCR和蛋白免疫印迹技术验证Twist1对Fox M1的调控作用。结果:Twist1、Fox M1在上皮性卵巢癌组织中的阳性表达率分别是71.4%(40/56)、78.6%(44/56),明显高于正常卵巢组织,差异有统计学意义(P<0.05);且其在上皮性卵巢癌中的表达显著相关(r=0.896,P<0.01);Twist1可以结合并激活Fox M1启动子(P<0.05);上调Twist1表达可以激活Fox M1在卵巢癌细胞中的表达(P<0.05),而干扰Twist1后可以下调Fox M1的表达(P<0.05)。结论:Twist1参与调控增殖相关转录因子Fox M1在卵巢癌中的表达,可能是一个潜在的治疗靶点。
Objective: To investigate the expression of Twist1 regulatory transcription factor Fox M1 in epithelial ovarian cancer. Methods: Immunohistochemical SP method was used to detect the expression of Fox M1 and Twist1 in epithelial ovarian cancer tissues. Spearman’s rank correlation analysis was used to detect the expression of Fox M1 and Twist1 in epithelial ovarian cancer tissues. Dual luciferase reporter assay was used to detect Twist1 On the regulation of Fox M1; Real-time quantitative RT-PCR and Western blotting to verify the role of Twist1 on Fox M1. Results: The positive rates of Twist1 and Fox M1 in epithelial ovarian cancer were 71.4% (40/56) and 78.6% (44/56), respectively, which were significantly higher than those in normal ovarian tissue (P < 0.05). The expression of Twist1 in epithelial ovarian cancer was significantly correlated (r = 0.896, P <0.01). Twist1 could bind and activate Fox M1 promoter (P <0.05) (P <0.05). However, the expression of Fox M1 was down-regulated after Twist1 interference (P <0.05). Conclusion: Twist1 is involved in the regulation of proliferation-related transcription factor Fox M1 in ovarian cancer, which may be a potential therapeutic target.