论文部分内容阅读
目的:研究前列腺癌组织及细胞株中丝氨酸-苏氨酸激酶11(liver kinase B1/serine-threonine kinase 11,LKB1)、转化生长因子-β1(transforming growth factor-β1,TGF-β1)的表达情况,探讨LKB1表达在前列腺癌发生演进中的作用。方法:用免疫组化SP法、Western blot和RT-PCR检测LKB1及TGF-β1在前列腺增生和前列腺癌组织,以及2种细胞株(正常前列腺上皮RWPE-1,前列腺癌细胞株PC-3)中的蛋白和m RNA表达情况。结果:前列腺癌组织中LKB1、TGF-β1的阳性表达率分别为55%、70%,前列腺增生组织中LKB1、TGF-β1的阳性表达率分别为83.3%、40%,前列腺癌组织中LKB1的表达显著低于前列腺增生组织(P<0.05),前列腺癌组织中TGF-β1的表达明显高于前列腺增生组织(P<0.05),LKB1和TGF-β1在前列腺癌中的表达呈负相关(r=-0.373,P=0.018);前列腺增生组织中LKB1的蛋白和m RNA表达水平明显高于前列腺癌组织(P<0.01),前列腺癌组织中TGF-β1的蛋白和m RNA表达水平显著高于前列腺增生组织。免疫细胞化学、Western blot、RT-PCR结果显示:LKB1在人前列腺上皮细胞株RWPE-1中的蛋白及m RNA表达水平明显高于前列腺癌细胞株PC-3(P<0.01);TGF-β1在前列腺癌细胞株PC-3中的蛋白及m RNA表达水平明显高于人前列腺上皮细胞株RWPE-1(P<0.01)。结论:LKB1在前列腺癌中表达下调,而TGF-β1在前列腺癌中过表达,LKB1在前列腺癌的发生过程中可能起着抑制TGF-β1的作用;对LKB1和TGF-β1相互作用机制的深入探讨有可能使其成为研究前列腺癌发生进展的分子机制新靶点。
Objective: To investigate the expression of LKB1 and TGF-β1 in prostate cancer tissues and cell lines , To explore the role of LKB1 expression in the evolution of prostate cancer. Methods: Immunohistochemical SP method, Western blot and RT-PCR were used to detect the expression of LKB1 and TGF-β1 in prostatic hyperplasia and prostate cancer tissues, as well as two cell lines (normal prostate epithelium RWPE-1 and prostate cancer cell line PC-3) In the protein and m RNA expression. Results: The positive rates of LKB1 and TGF-β1 in prostate cancer tissues were 55% and 70%, respectively. The positive rates of LKB1 and TGF-β1 in benign prostatic hyperplasia tissues were 83.3% and 40% (P <0.05). The expression of TGF-β1 in prostate cancer tissues was significantly higher than that in benign prostatic hyperplasia tissues (P <0.05). The expression of LKB1 and TGF-β1 in prostate cancer tissues was negatively correlated = -0.373, P = 0.018). The protein and mRNA expression of LKB1 in benign prostatic hyperplasia was significantly higher than that in prostatic cancer (P <0.01), and the protein and mRNA expression of TGF-β1 in prostate cancer was significantly higher than that in prostatic cancer Prostatic hyperplasia tissue. The results of immunocytochemistry, Western blot and RT-PCR showed that the expression of LKB1 protein and m RNA in RWPE-1 cells was significantly higher than that in PC-3 prostate cancer cells (P <0.01) The expression of protein and m RNA in prostate cancer cell line PC-3 was significantly higher than that in human prostate epithelial cell line RWPE-1 (P <0.01). CONCLUSION: LKB1 is downregulated in prostate cancer and TGF-β1 is overexpressed in prostate cancer. LKB1 may play a role in inhibiting the expression of TGF-β1 during the development of prostate cancer. The mechanism of LKB1 and TGF-β1 interaction Probable to make it a new target for the study of molecular mechanisms of prostate cancer progression.