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浙江省 1996～ 1999年急性弛缓性麻痹 (AFP)病例粪便标本中非脊髓灰质炎 (脊灰 )肠道病毒 (NPEV)的平均分离率为 11 2 %。在 6 7株NPEV中 ,柯萨奇病毒 (Coxsackievirus ,Cox )B组 2 1株 ,占 31 3% ;埃可病毒 (EntricCy topothicHumanOrphanviruses ,ECHO) 2 5株 ,占 37 4% ;不能确定型别 2 1株 ,占 31 3%。NPEV按月分布显示 ,Cox B病毒高峰期在 5～ 7月 ,在 1～ 3、10、12月未分离到 ;ECHO病毒全年均有 ,高峰期在 8～ 11月。Cox B病毒在Hep - 2、RD细胞上平均滴度分别为 10 7 2 和 10 4 6TCID50 /ml,ECHO病毒则分别为 10 3 1和 10 6 9TCID50 /ml。部分不能确定型别的病毒除了能在RD、Hep - 2细胞生长外 ,还能在L2 0B细胞上生长 ,其细胞病变与“腺病毒”相类似 ,但与脊灰病毒在L2 0B细胞上的细胞病变效应明显不同。
The average isolation rate of non-poliomyelitis (polio) enterovirus (NPEV) in stool specimens of acute flaccid paralysis (AFP) cases in Zhejiang Province from 1996 to 1999 was 112%. Among 6 7 strains of NPEV, 21 strains of Coxsackievirus (Cox) group B accounted for 31.3%, and 25 strains of Enriched Crohn’s disease (ECHO) accounted for 37.4%. Type 2 1 strain, accounting for 31.3%. The monthly distribution of NPEV showed that the peak period of Cox B virus was between May and July, and it was not isolated in January, March, October and December. ECHO virus was found all year round with a peak in August to November. The average titers of Cox B virus on Hep - 2 and RD cells were 10 7 2 and 10 4 6 TCID50 / ml respectively, and that of ECHO virus was 10 3 1 and 10 6 9 TCID50 / ml, respectively. In addition to being able to grow on RD and Hep - 2 cells, some of the undetectable viruses also grew on L2 0B cells whose cytopathicity was similar to that of “adenovirus” but not to that of poliovirus on L2 0B cells The cytopathic effect is significantly different.