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目的预测热带无爪螨主要变应原Blo t 21的抗原表位。方法利用DNAStar Protean软件分析热带无爪螨主要变应原Blo t 21的二级结构和表面特性,如理化性质、亲水性、表面可及性、柔韧性等,预测Blo t 21蛋白的B抗原表位和T抗原表位,同时利用Bepi Pred在线分析预测其可能的B细胞抗原表位。结果 Blo t 21蛋白的二级结构以α螺旋为主,存在7个潜在的B抗原表位位点,可能的B抗原表位区域有:2-11,19-28,35-54,55-70,72-83,96-112,123-129氨基酸残基或其附近。含有8个潜在的T细胞抗原表位区域:13-17,22-31,37-46,51-64,69-75,80-84,95-99,102-105氨基酸残基或其附近。结论应用Protean软件和Bepi Pred在线预测了热带无爪螨主要变应原Blo t 21的抗原表位,为进一步研究Blo t 21变应原的结构与功能,研制针对热带螨的预防性和治疗性疫苗、开发相应的诊断试剂盒奠定了基础。
Objective To predict the epitope of Blo t 21, a major allergen in tropical clawed mites. Methods The secondary structure and surface properties of Blo t 21, a major allergen of Acarus tropicalis, were analyzed by DNAStar Protean software, such as physicochemical properties, hydrophilicity, surface accessibility, flexibility and so on, to predict the B antigen of Blo t 21 protein Epitopes and T antigen epitopes, while using Bepi Pred online analysis of its possible B cell epitopes. Results The secondary structure of Blo t 21 protein was α helix, and there were 7 potential B antigen epitopes. The possible B epitopes were 2-11, 19-28, 35-54 and 55- 70, 72-83, 96-112, 123-129 amino acid residues or their vicinity. Contains eight potential T cell epitope regions: 13-17, 22-31, 37-46, 51-64, 69-75, 80-84, 95-99, 102-105 amino acid residues or their vicinity. Conclusions Proteomics software and Bepi Pred were used to predict the epitope of Blo t 21, the major allergen of T. mongolica. In order to further study the structure and function of Blo t 21 allergen, the preventive and curative effects against tropical mite Vaccine, the development of the appropriate diagnostic kits laid the foundation.