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目的探讨肌动蛋白相关蛋白复合体2/3(actin-related protein complex 2/3,ARPC2/3)在宫颈鳞癌中的表达及其临床意义。方法制备40例维吾尔族妇女子宫颈癌及15例正常宫颈组织中总RNA,采用RT-PCR法分析ARPC2/3基因的mRNA表达水平,同时采用免疫组化SP法检测其蛋白表达。结果 (1)维吾尔族妇女子宫颈癌组织及正常宫颈组织中ARPC2/3基因及蛋白阳性率分别为63%~65%及20%~27%(P<0.005)表达差异有显著性。(2)ARPC2/3基因及其蛋白在维吾尔族子宫颈癌不同临床分期及不同分化程度组织中表达差异无显著性(P>0.05),但是在维吾尔族妇女子宫颈癌淋巴结转移阳性组中表达率明显高于淋巴阴性组,差异有显著性(P<0.05)。结论 ARPC2/3在维吾尔族宫颈鳞癌中高表达,对宫颈癌的诊断及预后判断有重要的参考价值。
Objective To investigate the expression of actin-related protein complex 2/3 (ARPC2 / 3) in cervical squamous cell carcinoma and its clinical significance. Methods The total RNA was extracted from 40 Uighur women with cervical cancer and 15 normal cervical tissues. The mRNA expression of ARPC2 / 3 gene was analyzed by RT-PCR. The protein expression of ARPC2 / 3 was detected by immunohistochemistry. Results (1) The positive rates of ARPC2 / 3 gene and protein in Uighur women were 63% ~ 65% and 20% ~ 27% (P <0.005), respectively. (2) There was no significant difference in the expression of ARPC2 / 3 gene and its protein in Uygur cervical cancer with different clinical stage and different differentiation degree (P> 0.05), but it was expressed in Uygur cervical cancer patients with lymph node metastasis The rate was significantly higher than that in lymphoid negative group (P <0.05). Conclusion ARPC2 / 3 is highly expressed in Uigur cervical squamous cell carcinoma, which has important reference value for the diagnosis and prognosis of cervical carcinoma.