论文部分内容阅读
目的:探讨microRNA-205(miRNA-205)对人喉癌细胞株Hep-2细胞增殖的影响。方法:实时定量PCR方法检测27例喉癌组织及癌旁正常组织miRNA-205的表达水平,Western blot方法检测喉癌组织及癌旁正常组织PTEN蛋白表达;将miRNA-205inhibitors及miRNA-205mimics转染至Hep-2细胞,Western blot方法检测转染后PTEN蛋白表达的变化,并通过CCK-8法测定Hep-2细胞增殖能力的变化。结果:miRNA-205在喉癌组织中的表达量较癌旁组织明显升高(P<0.01),PTEN蛋白在喉癌组织中较癌旁组织表达明显降低(P<0.01);转染miRNA-205inhibitor的细胞增殖能力与对照组相比显著降低,细胞内PTEN蛋白表达亦明显升高(P<0.01),转染miRNA-205mimics的细胞增殖能力与对照组相比显著升高,细胞内PTEN蛋白水平明显降低(P<0.01)。结论:miRNA-205可能通过调节PTEN的表达促进喉癌细胞Hep-2的增殖。
Objective: To investigate the effect of microRNA-205 (miRNA-205) on the proliferation of human laryngeal carcinoma cell line Hep-2. Methods: Real-time quantitative PCR was used to detect the expression of miRNA-205 in 27 cases of laryngeal carcinoma and adjacent normal tissues. Western blot was used to detect the expression of PTEN in laryngeal carcinoma tissues and adjacent normal tissues. Transfection of miRNA-205inhibitors and miRNA-205mimics To Hep-2 cells, the expression of PTEN protein was detected by Western blot. The proliferation of Hep-2 cells was determined by CCK-8 assay. Results: The expression of miRNA-205 in laryngeal carcinoma tissues was significantly higher than that in adjacent normal tissues (P <0.01), and the expression of PTEN protein in laryngeal carcinoma tissues was significantly lower than that in paracancerous tissues (P <0.01) The proliferation of 205inhibitor was significantly lower than that of the control group, and the expression of PTEN protein was also significantly increased (P <0.01). The proliferation of miRNA-205mimics transfected cells was significantly higher than that of the control group. The intracellular PTEN protein The level was significantly lower (P <0.01). Conclusion: miRNA-205 may promote the proliferation of laryngeal carcinoma Hep-2 cells by regulating PTEN expression.