论文部分内容阅读
本文收集广州、中山县等鼻咽癌高发区的鼻咽癌病人和正常人的血清样本,应用免疫荧光和免疫酶标技术检测EB病毒壳抗原(VCA)、早期抗原(EA)的IgA或IgG型抗体以及核抗原(EBNA)抗体水平。 VCA与EA两者无论是IgA或IgG型抗体都有高度相关关系,而EBNA抗体与VCA或EA抗体的出现无相关性。VCA与EA的相关系数(γ)在0.265～0.731之间,表示两者有作常显著的相关关系(P值<0.01或0.001)。相反地,当EBNA抗体显著升高时,VCA和EA抗体可以不出现或抗体水平很低,EBNA抗体滴度与VCA或EA抗体滴度的增加无平行关系,二者间统计学上无显著意义。 上述检测结果表明:多种抗EB病毒抗体的检测方法的综合应用,不仅提高了鼻咽癌早期诊断的阳性率,而且可以降低由单一种EBV抗体检测方法所造成的假阴性。 本文还讨论了淋巴细胞亚型(B或T)与EBNA、VCA和EA三种抗体的相互关系,特别是T细胞功能与EBNA抗体水平的关系。
In this paper, serum samples from patients with nasopharyngeal carcinoma and normal persons in high incidence areas of nasopharyngeal carcinoma in Guangzhou and Zhongshan counties were collected. Immunofluorescence and enzyme-linked immunosorbent assay were used to detect Epstein-Barr virus envelope antigen (VCA), early antigen (EA) IgA or IgG Type antibodies and nuclear antigen (EBNA) antibody levels. There was a high correlation between VCA and EA, both IgA and IgG antibodies, whereas there was no correlation between EBNA and VCA or EA antibodies. The correlation coefficient (γ) between VCA and EA ranged from 0.265 to 0.731, indicating that there was a significant and often significant correlation between the two (P <0.01 or 0.001). Conversely, when EBNA antibodies were significantly elevated, VCA and EA antibodies were either absent or low, EBNA antibody titers were not paralleled by increases in VCA or EA antibody titers, with no statistically significant difference between the two . The above test results show that the combination of multiple anti-Epstein-Barr virus antibodies not only improves the positive rate of early diagnosis of nasopharyngeal carcinoma but also can reduce the false-negative caused by a single antibody detection method of EBV. This article also discusses the relationship between lymphocyte subsets (B or T) and EBNA, VCA and EA antibodies, specifically the relationship between T cell function and EBNA antibody levels.