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目的通过对中国脊髓灰质炎(脊灰)实验室网络(China Polio Laboratory Network,CPLN)开展细胞系支原体检测(Cell mycoplasma detection,CMD),评价CMD在细胞质量控制中的作用,探讨其与脊灰病毒分离率的联系。方法采用巢式聚合酶链反应(Nested polymerase chain reaction,n PCR)法对CPLN常规使用的RD、L20B细胞系进行支原体检测。结果 2013年8月至2016年8月,在CPLN中除西藏外的30个省级脊灰实验室已正式开展CMD,并按时向国家脊灰实验室上报结果。CMD结果显示,30个省级脊灰实验室所用的每个批次的L20 B和RD细胞,支原体检测结果均为阴性。结论三年间CPLN 30个省级脊灰实验室用于脊灰病毒分离的细胞系均未出现支原体污染,细胞系质量正常且稳定;CMD作为CPLN细胞质量控制的重要组成部分,为CPLN运转质量提供可靠的数据支撑。
OBJECTIVE: To evaluate the role of CMD in cell quality control by conducting cell mycoplasma detection (CMD) on the China Polio Laboratory Network (CPLN) Virus isolation rate of the link. Methods Nested polymerase chain reaction (n PCR) was used to detect mycoplasma in RD and L20B cell lines routinely used in CPLN. Results From August 2013 to August 2016, 30 polio laboratories except Tibet in the CPLN were formally launched CMD, and the results were reported to the National Polio Laboratory on time. CMD results showed that mycoplasma test results were negative for each batch of L20 B and RD cells used in the 30 provincial polio laboratories. CONCLUSION: Mycoplasma contamination was not observed in the poliovirus isolated cell lines of 30 provincial poliovirus laboratories of CPLN in 30 years. The quality of cell lines was normal and stable. As an important part of CPLN cell quality control, CMD provided the quality of CPLN Reliable data support.