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目的:观察灯盏乙素对痴呆模型大鼠氧化应激水平和神经细胞凋亡的影响,探讨其对阿尔茨海默病(Alzheimer’sdisease,AD)的治疗作用及其机制。方法:Wistar大鼠42只,随机分为5组,即正常对照组、假手术组、模型组、灯盏乙素处理组、及脑复康处理组。用双侧脑室注射β淀粉样蛋白(Aβ25-35)并联合腹腔注射D-半乳糖方法建立痴呆大鼠模型;用Morris水迷宫方法检测大鼠学习记忆能力;光镜下观察大鼠大脑皮质及海马形态学改变,尼氏染色法观察海马CA1区尼氏小体改变;用黄嘌呤氧化酶法测定大鼠脑组织超氧化物歧化酶(SOD)的活性;生物化学法测定单胺氧化酶(MAO)的活性;用流式细胞术检测大鼠脑神经细胞凋亡情况。结果:模型组大鼠学习记忆能力下降,脑组织海马区出现病理形态改变,SOD活性降低,MAO活性升高,神经细胞凋亡发生率升高;经灯盏乙素治疗后大鼠学习记忆能力改善,脑组织形态学病理改变减轻,SOD活性上升、MAO活性降低,神经细胞凋亡发生率显著下降;灯盏乙素处理组与脑复康处理组相比未见明显差异。结论:灯盏乙素可能通过降低氧化应激水平及对抗凋亡发生等在AD的治疗中发挥作用。
Objective: To observe the effect of scutellarin on oxidative stress and apoptosis of neuron in dementia model rats, and to explore its therapeutic effect on Alzheimer’s disease (AD) and its mechanism. Methods: Forty-two Wistar rats were randomly divided into five groups: normal control group, sham operation group, model group, scutellarin treatment group and nalfufang treatment group. Rat models of dementia were established by intracerebroventricular injection of amyloid-β (Aβ25-35) combined with intraperitoneal injection of D-galactose. Morris water maze test was used to evaluate learning and memory abilities of rats. Cerebral cortex and The changes of hippocampal morphology and Nissl staining were observed by Nissl staining. The activity of superoxide dismutase (SOD) in rat brain was measured by xanthine oxidase method. The levels of monoamine oxidase (MAO) The apoptosis of neurons in rat brain was detected by flow cytometry. Results: The learning and memory abilities of the model group decreased, the pathological changes of the hippocampus of the model group were observed, the activity of SOD decreased, the activity of MAO increased and the rate of neuronal apoptosis increased. The learning and memory ability of the model group was improved , The histopathological changes of brain tissue were alleviated, the activity of SOD increased, the activity of MAO decreased and the incidence of neuronal apoptosis decreased significantly. There was no significant difference between Scutellarin treatment group and Naofukang treatment group. Conclusion: Scutellarin may play a role in the treatment of AD by reducing the level of oxidative stress and anti-apoptosis.