从５００个随机引物中筛选出３０个在玉米上具有较好多态性的ＲＡＰＤ随机引物，并进一步筛选确定了适合我国主要玉米杂交种及其双亲的特异引物，建立了相应的ＤＮＡ指纹图谱。杂交种的ＤＮＡ指纹图谱表现为父、母本双亲带型的互补，均未出现互补带型之外的新带型。应用ＲＡＰＤ技术获得ＤＮＡ指纹图谱的方法可以简便、快捷、经济、准确地鉴别玉米杂交种子纯度及真伪。目前已进行了上百份的检测实践，无一差错 Thirty RAPD random primers with good polymorphism in maize were screened from 500 random primers, and the specific primers suitable for the main maize hybrids and their parents were screened out and the corresponding DNA fingerprints were established. The DNA fingerprinting of hybrids showed the complementarity of parental parental banding patterns, and no new banding patterns were found except for complementary banding patterns. The method of obtaining DNA fingerprinting using RAPD technology can identify the purity and authenticity of the corn hybrid seeds easily, rapidly, economically and accurately. At present, hundreds of tests have been conducted without any mistakes