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用HPLC法测定了心肌炎片中黄连素的含量,使用RP-18色谱柱,流动相为乙腈-磷酸盐缓冲液(pH5.2)-甲醇(5:6:1)。对供试品采用超声波振荡提取,再经氧化铝柱层析除杂后的溶液,进样分析。
The content of berberine in the myocarditis tablets was determined by HPLC. The RP-18 column was used. The mobile phase was acetonitrile-phosphate buffer (pH 5.2)-methanol (5:6:1). The test sample was extracted by ultrasonic vibration, and the solution was removed by the alumina column chromatography and analyzed.