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用抗FOS免疫组化技术,观察胃肠道伤害性刺激诱导大鼠孤束核、视上核、室旁核内c-fos的表达,并结合抗TH免疫双重染色技术,探讨孤束核内儿茶酚胺能神经元与FOS蛋白的关系.结果表明:FOS阳性细胞主要分布于孤束核的连合亚核、内侧亚核以及背侧周边区,说明孤束核是内脏伤害性信息初级传入冲动的直接反应区.在下丘脑内主要分布于视上核和室旁核,提示视上核和室旁核在内脏伤害性刺激的传递中起中继作用.在双标切片中,FOS/TH双标细胞集中分布于孤束核的内侧亚核和背侧周边区,说明孤束核内儿茶酚胺能神经元也参与内脏伤害性刺激反应.
The anti-FOS immunohistochemistry was used to observe the expression of c-fos in the nucleus tractus solitarius, supraoptic nucleus and paraventricular nucleus of rats induced by gastrointestinal noxious stimulation. Combined with anti-TH immune double staining technique, Catecholaminergic neurons and FOS protein.The results showed that FOS positive cells were mainly distributed in the commissural subnucleus, medial subnucleus and dorsal peripheral region of the nucleus tractus solitarius, indicating that the solitary tract nucleus is the primary afferent impulse of visceral nociceptive information In the hypothalamus mainly in the supraoptic and paraventricular nucleus, suggesting that the supraoptic nucleus and paraventricular nucleus in the transmission of visceral nociceptive stimuli play a relay role in double-labeled sections, FOS / TH double-labeled cells Concentrated in the medial subnucleus and dorsal marginalis of the nucleus tractus solitarius, suggesting that catecholaminergic neurons in the nucleus tractus solitarii are also involved in visceral noxious stimulation.