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目的:为了观察中药苏合香对联用药西药舒必利血药与药脑浓度的影响,探讨苏合香开窍作用与增加胃肠屏障与血脑屏障透过性的关系,并研究苏合香与舒必利联用的相互作用。方法:大鼠连续口服用药1周后,进行脑部与颈部安装探针手术,平衡1 h后,利用血微透析法与脑微透析法分别在(30,60,90,120,150,180 min)各时间段采集大鼠右心房内血液中与脑海马组织中的透析液,利用反相高效液相荧光色谱系统检测样品中舒必利的含量,利用统计学方法比较2组给药后脑中及血液中的舒必利含量。结果:舒必利合用苏合香组的大鼠脑中和血中舒必利浓度明显高于单用舒必利组,单用舒必利组大鼠血中和脑中舒必利的浓度比值为1∶0.2,舒必利与苏合香合用组血脑比增加为1∶0.3,与单用舒必利组相比,舒必利与苏合香合用组的舒必利脑浓度上升39%,血浓度上升69%。结论:苏合香能够明显增加舒必利在大鼠脑中和血中的浓度,说明苏合香能够促进舒必利透过胃肠屏障与血脑屏障,该研究从药理学角度揭示了苏合香“开窍”的机制是促进了胃肠屏障及血脑屏障通透性增加。
OBJECTIVE: To investigate the effect of traditional Chinese medicine Su-Xiang on the concentration of sulpiride and its drug concentration in Chinese medicine, and to explore the relationship between Su-shan-xiao-qiao and increasing permeability of the gastro-intestinal barrier and blood-brain barrier, and to study the interaction between Su-xiang and sulpiride. Methods: After a week of continuous oral administration, rats were subjected to probe installation of the brain and neck. After equilibrating for 1 hour, the blood microdialysis and brain microdialysis were used to detect the changes in time (30, 60, 90, 120, 150 and 180 min) The dialysate in the right atrium of the rat and hippocampus were collected, and the contents of sulpiride in the samples were determined by RP-HPLC. The sulpiride contents in the brain and blood were compared by statistical methods . Results: The sulpiride concentration in the brain of rats treated with sulpiride combined with Su-He-Xiang group was significantly higher than that of sulprofen alone group. The concentration ratio of sulpiride in blood and brain was only 1: 0.2 in sulprofen group alone and sulpiride Compared with the single sulpiride group, sulpiride combined with sulpiride increased brain concentration by 39% and blood concentration increased by 69%. CONCLUSION: Su-Hexiang can significantly increase the concentration of sulpiride in the brain and blood of rats, indicating that su-Hexiang can promote the penetration of sulpiride through the gastro-intestinal barrier and the blood-brain barrier. The mechanism of Su-xiang from the pharmacological point of view Promote gastrointestinal barrier and increased permeability of the blood-brain barrier.