总结了人体上皮细胞的原代培养方法，详述了组织块培养法，单细胞分离培养法、成纤维细胞清除法及其关键技术，如以冷胰蛋白酶消化法制备各种上皮细胞悬液，用机械刮除加酶消法清除成纤维细胞。本资料有助于基础医学研究及在临床应用中获得理想的研究材料。 The primary culture methods of human epithelial cells were summarized. The tissue culture method, single cell culture method, fibroblast clearance method and its key technologies were described in detail. For example, various epithelial cell suspensions were prepared by cold trypsin digestion, Use mechanical scraping plus enzyme elimination method to remove fibroblasts. This information is helpful for basic medical research and obtaining ideal research materials in clinical application.