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目的:观察脊髓损伤后CSPGs的表达及其与GFAP的关系。方法:成年雄性SD大鼠25只,随机分为对照组和损伤组,损伤组分脊髓挤压损伤后0h、72h、1w、4w组,运用免疫荧光双重染色方法观察CSPGs与GFAP的表达。结果:挤压伤后损伤部位的CSPGs和GFAP的表达均增高,但二者的变化趋势并不一样。其中CSPGs从损伤后表达开始增高,此后一直增加,并在1w至4w时逐渐稳定,主要分布逐渐集中于损伤部位;星形胶质细胞的免疫反应也逐渐增加,其分布逐渐集中于损伤区域的边缘,逐渐形成胶质瘢痕界膜。损伤1w至4w,损伤区域内几乎没有了星形胶质细胞表达,但仍留有大量的CSPGs。结论:早期抑制星形胶质细胞分泌CSPGs,可以防止在损伤部位沉积大量的CSPGs,从而减小其对再生纤维的抑制作用。
Objective: To observe the expression of CSPGs after spinal cord injury and its relationship with GFAP. Methods: Twenty-five adult male Sprague-Dawley rats were randomly divided into control group and injury group. The injury components were observed at 0h, 72h, 1w and 4w after spinal cord injury. The expressions of CSPGs and GFAP were observed by immunofluorescence double staining. Results: The expression of CSPGs and GFAP increased after crush injury, but the trend of change was not the same. The expression of CSPGs increased from the beginning of injury, then increased gradually, and gradually stabilized from 1w to 4w. The distribution of CSPGs mainly concentrated on the injury site. The immune responses of astrocytes also gradually increased, and their distribution gradually concentrated in the lesion area Edge, gradually forming glial scar membrane. Injured 1w to 4w, almost no astrocyte expression in the injured area, but still retain a large number of CSPGs. CONCLUSIONS: Early inhibition of CSPGs secretion by astrocytes prevents the deposition of large numbers of CSPGs at the site of injury, thereby reducing their inhibitory effect on regenerated fibers.