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本实验将血液与脑脊液(CSF)孵育5天,5-HT无明显减少。5-HT对基底动脉与股动脉均具有强烈的收缩作用(+++)。电镜检查产生痉挛动脉的内皮细胞与中膜平滑肌细胞,发现有空泡变性。5-HT引起痉挛动脉壁的6-酮-PGF_1、血栓烷B_2(TXB_2)、前列腺素E_2(PGE_2)、过氧化脂质(LPO)均无变化。放射免疫测定发现正常情况下,基底动、静脉6-酮-PGF_1含量明显高于其他部位血管,而TXB_2、PGE_2各血管无明显差异。氧合血红蛋白(OxyHb)可诱发基底动脉强烈收缩(+++)。电镜检查痉挛动脉的内皮细胞与中膜平滑肌细胞,见有空泡变性;LPO升高,6-酮-PGF_1下降。但OxyHb不诱发股动脉的收缩(一)或只引起轻微收缩(±);LPO升高,6-酮-PGF_1无变化;电镜检查动脉壁正常。应用不同的自由基清除剂与OxyHb混合,然后作用于基底动脉。只有羟自由基(·OH)清除剂甘露醇有缓解脑血管痉挛的作用。
This experiment will blood and cerebrospinal fluid (CSF) incubated for 5 days, 5-HT no significant reduction. 5-HT has a strong contractile effect on basilar and femoral arteries (+++). Electron microscopy to produce spasm arteries of endothelial cells and smooth muscle cells in the medium, found vacuolar degeneration. There were no changes in 5-HT-induced arterial wall 6-keto-PGF_1, TXB_2, PGE 2, and LPO. Radioimmunoassay found that under normal circumstances, basal animals, venous 6-keto-PGF1 levels were significantly higher than other parts of the blood vessels, and TXB_2, PGE_2 of blood vessels no significant difference. Oxygenated hemoglobin (OxyHb) can induce strong contraction of the basilar artery (+++). Electron microscopy spasm artery endothelial cells and smooth muscle cells in the media, see the vacuolar degeneration; LPO increased, 6-keto-PGF_1 decreased. However, OxyHb did not induce femoral artery contraction (a) or only slight contraction (±); LPO increased, 6-keto-PGF_1 did not change; electron microscopy of normal artery wall. Different free radical scavengers were mixed with OxyHb and then applied to the basilar artery. Only hydroxyl free radical (· OH) scavenger mannitol can relieve cerebral vasospasm.