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目的研究原发性肝癌组织转化生长因子β1（TGF-β1）和增殖细胞核抗原（PCNA）的表达和意义．方法应用免疫络化染色采用链菌素亲生物素过氧化酶连接法（S-P法）检测我院1990／1996外科住院患者手术经病理证实50例原发性肝癌及30例肝硬变石蜡包埋组织的TGF-β1和PCNA的表达进行同步检测，并结合临床及血清AFP值进行统计学分析．结果肝癌细胞TGF-β1表达增强，TGF-β1着色于细胞浆内呈棕黄色细颗粒状，阳性细胞呈弥漫性或灶状分布，其阳性率84％（42／50）显著高于肝硬变组40％（12／30）和癌旁组织46％（23／50）（P＜0．05）．肝癌细胞PCNA表达显著增强，PCNA染色局限在细胞核内，呈棕黄色颗粒状，PCNA阳性反应细胞核形态规则，多为圆形或椭圆形，大小较一致，阳性率平均为28．4％±18．7％，而肝硬变组3．1％±1．8％，癌旁组织为4．30％±．6％（P＜0．01）．观察了PCNA表达与细胞分化程度的关系，根据细胞分化程度，分为好、中、差三级，其中分化Ⅰ级22例，PCNA阳性表达率为15．3％±9．3％，Ⅱ级18例，为30．7％±14．5％，Ⅲ级10例，为51．0％±14．1％，各组间有显著差异（P＜0．01）、细胞分化愈差，PCNA值愈高同时比较了TGF-β1和PCNA表达与血AFP水平的关系，结果看到TGF-β1表达与血AFP无相关?
Objective To study the expression and significance of transforming growth factor-β1 (TGF-β1) and proliferating cell nuclear antigen (PCNA) in primary hepatocellular carcinoma (HCC). Methods Immunohistochemical staining was performed to detect 50 cases of primary liver cancer and 30 cases of hepatic hard paraffin embedding in our hospital from 1990 to 1996 by immunohistochemical staining using streptavidin-biotin peroxidase-linked immunosorbent assay (SP method). The expression of TGF-β1 and PCNA was detected simultaneously in the tissues, and the clinical and serum AFP values were analyzed statistically. Results The expression of TGF-β1 in hepatocellular carcinoma cells was enhanced. The TGF-β1 was stained in the cytoplasm with brownish yellow fine particles. The positive cells showed diffuse or focal distribution. The positive rate was 84% (42/50) significantly higher than that of cirrhosis. Group 40% (12/30) and paracancerous tissue 46% (23/50) (P<0.05). The expression of PCNA in hepatocellular carcinoma cells was significantly enhanced. PCNA staining was localized in the nucleus and showed brownish yellow granular morphology. PCNA positive reaction showed nuclear morphology, mostly round or oval, and the size was relatively uniform. The positive rate was 28.4%±18. 7%, while liver cirrhosis group 3.1% ± 1.8%, paraneoplastic tissue 4.30% ±. 6% (P<0.01). The relationship between the expression of PCNA and the degree of cell differentiation was observed. According to the degree of cell differentiation, it was divided into three grades: good, moderate, and poor. Of these, 22 were differentiated in grade I and the positive rate of PCNA was 15.3% ± 9.3%. In 18 cases, it was 30.7%±14.5%, and in grade III, it was 51.0%±14.1%. There were significant differences among groups (P<0.01), and the cell differentiation was worse. PCNA The higher the value, the more the relationship between the expression of TGF-β1 and PCNA and the level of blood AFP was compared. The results showed that there was no correlation between TGF-β1 expression and blood AFP.