论文部分内容阅读
从茶Camellia sinensis(L.)O.Kunize叶甲醇提取物中分离、鉴定了5个新的三萜皂苷foliathea- saponinⅠ～Ⅴ(1～5),并研究了它们对β-己糖胺酶在RBL-2H3细胞中释放的抑制作用。茶叶用甲醇回流提取3次,减压蒸干溶剂,取部分提取物用醋酸乙酯-水萃取,水相再用正丁醇提取,正丁醇溶部位经多种柱色谱法分离和精制,得到化合物1～5,均为无色细小结晶。化合物1 mp
Five new triterpenoid saponins foliathea-saponinⅠ ~ Ⅴ (1-5) were isolated and identified from the methanolic extracts of Camellia sinensis (L.) O.Kunize. Their effects on β-hexosaminidase Inhibition of release in RBL-2H3 cells. The tea leaves were extracted with methanol three times, and the solvent was evaporated under reduced pressure. A portion of the extract was extracted with ethyl acetate-water and the aqueous phase was extracted with n-butanol. The n-butanol-soluble fraction was separated and purified by various column chromatographies. Compounds 1 to 5 are obtained, all of which are colorless and fine crystals. Compound 1 mp