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目的观察褪黑素（ＭＴ）对吗啡（Ｍｏｒ）致依赖性的作用及机制分析。方法连续ｉｐＭｏｒ与纳络酮催促建立小鼠依赖模型，甩尾法，淋巴细胞增殖反应和脑啡肽（Ｍｅｔ－Ｅｎｋ）放免测定。结果Ｍｏｒ依赖组小鼠痛阈下降，跳跃次数增加，肛温降低，伴有胸腺细胞增殖能力的低下，Ｍｏｒ依赖小鼠的脑和血浆Ｍｅｔ－Ｅｎｋ亦明显降低。本文首次发现ｉｇＭＴ（３０、９０ｍｇ·ｋｇ－１×７ｄ）能明显提高依赖性小鼠的痛阈，减少跳跃次数，恢复其肛温，阻止Ｍｏｒ对淋巴细胞功能的抑制，并能维持脑、血浆Ｍｅｔ－Ｅｎｋ接近正常对照水平。结论ＭＴ具有对抗Ｍｏｒ致小鼠依赖性作用，其机制与维持内源性Ｍｅｔ－Ｅｎｋ水平有关。
Objective To observe the dependence and mechanism of melatonin (MT) on morphine. Methods Continuous ipMor and naloxone promoted the establishment of mouse dependent model, tail flick method, lymphocyte proliferation reaction and enkephalin (Met-Enk) radioimmunoassay. Results The mice in Mor dependent group had lower pain threshold, more jumps, lower rectal temperature, lower thymocyte proliferation, and decreased Met-Enk in Mor-dependent mice. It was the first time that igMT (30, 90 mg · kg-1 × 7 d) could significantly increase the pain threshold, reduce the number of jumps, restore the rectal temperature and prevent Mor inhibition of lymphocyte function, and maintain the brain and plasma Met-Enk approached normal control levels. Conclusion MT has the anti-mouse Mor-dependence effect and its mechanism is related to maintaining the level of endogenous Met-Enk.